BrightBox Next Gen Sequencing

What is the estimated starting concentration of your library?

For your library, we recommend:

Try 2 uL

Number Of Reactions

Helpful description

% Overage Required

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Volume of Library and Standards

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Component	Stock Concentration	Volume	Volume
Assay Master Mix	6X	1.7 uL	1.7
Sample	-	1 - 5 uL	-
dH₂O	-	Volume to make up	7.39
Final Reaction		10 uL	10.1

uL of Assay mix / water per well

Raw RFU Data

Corrected Data Post RFU - Pre RFU